Digital Refractometer for % Glucose by Weight Analysis
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The HI96803 portable refractometer converts the refractive index of a food sample to % glucose by weight. This conversion is based on the tables found in the ICUMSA Methods Book (International Commission for Uniform Methods of Sugar Analysis) that documents the changes in refractive index with temperature for a percent by weight glucose solution. The HI96803 is ideal for the analysis of fruits, energy drinks, puddings, soy milk, juices, jam, marmalade, honey, soups, jelly, tofu and condiments. The HI96803 is an easy to use tool for measuring sugar in food samples in the field or in the lab.
- Designed for food sugar analysis
- Temperature Compensation algorithms based on glucose solution
- 0 to 85% glucose by weight range with an accuracy of ± 0.2%
Making a % Standard Glucose Solution
To make a glucose Solution, follow the procedure below:- Place container (such as a glass vial or dropper bottle that has a cover) on an analytical balance.
- Tare the balance.
- Weigh out X grams of high purity glucose (CAS #: 50-99-7) directly into a beaker
- Add distilled or deionized water to the beaker so the total weight of the solution is 100g.
- Note: Solutions above 60% may need to be vigorously stirred or shaken and heated in a water bath to roughly 40°C (104°F). Remove solution when fructose has dissolved and allow to cool before use.
Example to prepare as 25% glucose solution:
| Glucose % (g/100g) | Glucose (g) | Water (g) | Total (g) |
| 25 | 25.000 | 75.000 | 100.000 |
Refractive Index
The HI96803 takes measurements based on a sample’s refractive index. Refractive index is a measurement of how light behaves as it passes through the sample. Depending on the sample’s composition, light will refract and reflect differently. By measuring this activity with a linear image sensor, the sample’s refractive index can be assessed and used to determine its physical properties such as concentration and density. In addition to the linear image sensor, the HI96803 uses an LED light, prism and lens to make measurement possible.
Variations in temperature will affect the density of a solution based on the compound that is present. In digital refractometry the use of temperature compensation is necessary for accurate results. The HI96803 contains a built-in temperature sensor and is programmed with temperature compensation algorithms in accordance with the ICUMSA Methods Book for a percent by weight sucrose solution.
Features-at-a-glance
- Calibrate with distilled or deionized water
- Sample size can be as small as 2 metric drops (100 μl)
- Samples automatically compensated for temperature variations
- Readings are displayed in approximately 1.5 seconds
- The dual-level LCD displays measurement and temperature readings simultaneously
- Easy to clean and corrosion-resistant
- Battery percent level remaining at startup and low battery indicator
- To conserve battery life the meter shuts off automatically after three minutes of non-use
- Easy to clean and corrosion-resistant
- Water resistant ABS plastic casing designed to perform under laboratory and field conditions.
Resources
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Specifications
Specification Name Detail SKU HI96803 Sugar Content Range 0 to 85% mass (% w/w glucose) Sugar Content Resolution 0.1 % mass Sugar Content Accuracy ±0.2% mass Temperature Range 0.0 to 80.0°C (32.0 to 176.0°F) Temperature Resolution 0.1°C (0.1°F) Temperature Accuracy ±0.3°C (±0.5°F) Automatic Shut-Off after three minutes of non-use Battery Type / Life 9V / approximately 5000 readings Enclosure Rating IP65 Dimensions 192 x 102 x 67 mm (7.6 x 4.01 x 2.6”) Weight 420 g (14.8 oz.) Refractometer Light Source yellow LED Refractometer Temperature Compensation automatic between 10 and 40°C (50 to 104°F) Minimum Sample Volume 100 μL (to cover prism totally) Sample Cell stainless steel ring and flint glass prism Measurement Time approximately 1.5 seconds Ordering Information Supplied with battery and instruction manual. Warranty 2 years - Product Manuals
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